Paeoniflorin Attenuates Myocardial Ischemia / Reperfusion Injury via Up-Regulation of Notch 1 ? Mediated Jagged1 Signaling

Abstract

Nasser Ghaly Yousif, Zahraa Younis, Fadhil G. Al-Amran, Maitham G. Yousif, Ahmed Altimim, Najah R. Hadi.

Ischemic heart disease represents one of the major reasons of death worldwide. Methods and materials: A total number of 48 mice were assigned to one of the following 4 experimental groups (n = 8 in each group); sham; control I/R; I/R + vehicle group and I/R + Paeoniflorin pretreated group treated with (5 mg/kg intraperitoneal dose of PF) 30 min before ischemia and underwent 30 minute of LAD ligation followed by 72 hours reperfusion. After analysis of cardiac function, the heart tissue and blood were collected and prepared for analysis. Results: The % FS was significantly higher at 3 days after surgery in Paeoniflorin treated mice than I/R control and vehicle treated mice. Paeoniflorin treated mice had lower levels of MCP-1 in myocardial tissue and plasma P<0.05. Furthermore, plasma myocardial injury marker (cTn-I) level is associated with over expression of pro-inflammatory mediators after I/R. Over expression of inflammatory mediators following I/R suppress LV function; Notch-1 and Jagged-1 protein are down-regulated following I/R; and the administration of Paeoniflorin attenuated chemokine and cytokines through up-regulation of Notch-1 and Jagged-1 activation signaling pathway leading to improved left ventricular function