Pluchea indica Extract as a Potential Source of Nutrition for Accelerate Wound Healing

Abstract

Vinna Kurniawati Sugiaman, Nur Qolbiyatun Nisyah, Niki Anisa, Natallia Pranata

In qualitative phytochemical test, beluntas leaves (Pluchea indica) extract has proved to have flavonoids. Flavonoids have the potential to be an antioxidant. This is a laboratory experimental study. The beluntas leaves that were used came from botanical garden Manoko Lembang, Balai Penelitian Tanaman Rempah dan Obat, Indonesian Agency for Agricultural Research and Development (IAARD), Ministry of Agriculture in Bandung, West Java, Indonesia and were determined at the Identification and Determination Laboratory, The School of Life Science and Technology, Institut Teknologi Bandung (ITB). The extraction of 70 % ethanol and the phytochemical test of beluntas leaves were conducted at Aretha Medika Utama Biomolecular and Biomedical Research Center. The determination of total flavonoid levels was carried out by the Quercetin standard using aluminum chloride solvents. The total flavonoid value was obtained from the average value of the measurements after three repetitions. Antioxidant activity was tested by DPPH (2,2-diphenyl-1-picrylhydrazil) Free Radical Scavenging Assay. Absorbance was measured using a UV-Vis spectrophotometer, at λ = 517 nm. In this test the extract was diluted serially, at concentrations of 200, 100, 50, 25, 12.5, 6.25, 3.125 μg / ml. Prestoblue cytotoxicity test was also conducted to determine the effect on viability fibroblast 3T3 BALB C. The beluntas leaves has a total flavonoid of 19.44 mg per 1 gram of ethanol extract. The antioxidant activity included in high category with the IC50 value of 21.53 μg/ml. This extract also classified have no cytotoxic activity.