Potential Impact of micro RNA-146a Gene Polymorphisms in Oxidative Stress of Diabetic Mellitus Type 1

Abstract

Fulla Abd Alsattar Alriyahee, Noora M. Hameed, Israa Harjan Mohsen, Mona N. Al-Terehi.

Micro-RNA is small non coding molecules discovered recently and it has main role in different cell process and its deregulation contributed in different diseases etiology, the present study aims to investigate effect of micro-RNA 146a gene polymorphism in oxidative stress in diabetes mellitus type 1 patients, samples and data collection implemented for fasting blood glucose (FBG), total antioxidant (TOT) and reactive oxygen species (ROS) in addition to DNA extraction for gene amplification and genotyping by single strand conformation polymorphism technique, the results of present study show significant elevation in FBG (194.29, 65.00 )and TOT (1.433, 0.566) for patient and control respectively, also significant differences in ROS level (4.848, 4.98). the genotyping show (A, B, C and D) genotypes, a significant differences in C and D (P 0.0004), C was found in all patients but didn’t appeared in control, D found in all control while didn’t found in patients, A found in 61.53% and 100% in patients and control in non –significant (p 0.0643), B found in 73.07% in DM1 patients and in all control. The effect of genotyping in oxidative stress shows all genotyping effect in ROS and total antioxidant level, A, B and C show significant differences in total antioxidant (P 0.017, 0.017, and 0.008), but no significant differences were observed in ROS for all genotyping. The present study concluded that the microRNA-146a associated with oxidative modulator in diabetes mellitus type 1.