Prevalence of virulence factors genes of Escherichia coli isolated from pregnant and non-pregnant women with urinary tract infection in Diyala Iraq

Abstract

Saba J. Jawad AL – Zubaidi

Uropathogenic Escherichia coli (UPEC) is an essential cause factor of complicated and uncomplicated urinary tract infections. It is in charge of approximately 80% of community gained and 50% of nosocomial infection. The current study included collection of one hundred and eighty samples among different cases (pregnant and non-pregnant women) by urinary tract infection in Al – Batoul Teaching Hospital in Baquba, during the period from November 2017to February 2018. All isolates were identification as E. coli by using Gram – staining. EMB agar and conformation in VITEK2 compact system. It was found 96 E. coli isolates; it was carried out to 38 E. coli isolates. 18 isolates from pregnant and 20 isolates from non – pregnant women. In this study , E.coli isolates were resistance against different type antibiotics , it was pipracillin 84.38% ; tetracycline 70.83% ; Rifampicin 63.54% ; Levofloxacine 59.37% ; ciprofloxacin 54.17% ; tobramycin 47.9% ; Augmentin 39.58% ; gentamicin 35.49% ; chloramphenicol 30.21% ; Imipenem 26.04% .The molecular detection of virulence factors genes shows the prevalence of genes fimH , papC , hlyA , sfaS , iutA , ibeA were 100% , 94.44% , 55.5% , 72.2% , 66.66% , 22.22% respectively in pregnant case according to the mentioned tract infection. The prevalence of gene fimH , papC , hlyA , sfaS , iutA , ibeA were 85% , 60% , 25% , 45% , 45% , 5% respectively in non-pregnant women with urinary tract infection . All the studied strains showed 16 of virulence gene patterns, among isolates from pregnant 12 patterns, on the other hand the isolates from non-pregnant showed diversity of genes patterns.