Protective role of Urtiea dicica on the pathological alteration induced by ethylene glycol in male rabbits

Abstract

Ibtisam N. Al-assaf, Ammar G. A.Al-Dabbagh, Razan Y.Q.Al-Abachi, Shihab A.Al-Bajari

Ethylene glycol (EG) is a colorless, odorless, sweet-tasting compound that is primarily used as antifreeze that is lethal when swallowed. Urtica dioica is used in the many countries as seasoning and as a herbal medicine (antioxidant). Purpose of the research was to determine the protecting function of U. dioica against the Ethylene glycol as toxicant in rabbits. Twenty-five rabbits were divided into five groups: (GI, control group) 5 untreated rabbits with ethylene glycol and (GII) 5 rabbits gave 0.75% ethylene glycol in drinking water for 30 days only, while (GIII, GIV and GV)15 rabbits gave 0.75% ethylene glycol in drinking water and U. dioica oral supplement (flavonoids, glycosides and alkaloids) extracts (100mg / kg bw) Two times daily for 30 days. Blood samples (plain tube & EDTA tube) were collected for clinical examination. EG induced a significant reduction (20%) in rabbits BW in G II compared to GI, GIII, GIV and GV. As a result, AST, ALT, ALKP, TBIL, MDA, SOD, urea, creatinine, globulin and monocytesine G II were markedly increased (P=0.05); meantime there was a markedly decrease (P=0.05) in whole Protein, albumin, ratio A / G, Gpx, CAT, GSH, BP, Hb, WBC and RBC. However, after treatment with U. dioica extracts, there was a significant reduction in AST, ALKP, TBIL, MDA, urea, creatinine and monocytes, while SOD, ALTno was significantly decreased. In the meantime, there has been a significant increase (P=0.05) in total protein, A / G ratio, Gpx, GSH, BP, Hb, WBC and RBC.