Protein Isolation from Sponge Niphates sp. as an Antibacterial and Antioxidant
Abstract
Warsidah, Masrianih, Mega Sari Juane Sofiana, Ikha Safitri, Ajuk Sapar, Anthoni B. Aritonang, Yuges Saputri Muttalib, Dzul Fadly.
Research on the antioxidant and antibacterial activity of the isolated protein of Niphates sp. sponge origin Spermonde aquatic of South Sulawesi had been conducted. Determination of antimicrobial activity was based on the formation of inhibition zones/cleared areas surrounding the isolated protein for two tested bacteria, i.e., Bacillus subtilis and Escherichia coli. The isolated protein from this sponge has the highest antimicrobial activity in E. coli bacterial assay by 14.21 mm zone of inhibition, while the inhibition zone of B. subtilis was 12.05 mm. After extra time for 24 hours of incubation, the medium of B. subtilis showed turbidity in the clear spot before. This indicated that the isolated protein covered the bacteriostatic ability against the tested bacteria. The antioxidant activity assay by DPPH free radical scavenging effect of isolated protein with some levels of saturation was determined. Following the test results indicated the antioxidant activity of isolated protein with scavenging effect against free radical DPPH possessed IC50 values from crude protein extract of 5.05 μg/mL, protein fraction 0 – 30% of 203.71 μg/mL, protein fraction 31 – 50% of 163.75 μg/mL, protein fraction 51 – 70% of 111.31 μg/mL, and protein fraction 71 – 90% amounted to 590.03 μg/mL. The results indicated that the sponge material's protein was potential as an antioxidant and antimicrobial, which can then be further research to several other biologic activities.
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| Pubmed Style , Masrianih , Sofiana MSJ, Safitri I, Sapar A, Aritonang AB, Muttalib YS, Fadly D. Protein Isolation from Sponge Niphates sp. as an Antibacterial and Antioxidant. SRP. 2020; 11(9): 518-521. doi:10.31838/srp.2020.9.76 Web Style , Masrianih , Sofiana MSJ, Safitri I, Sapar A, Aritonang AB, Muttalib YS, Fadly D. Protein Isolation from Sponge Niphates sp. as an Antibacterial and Antioxidant. http://www.sysrevpharm.org/?mno=13713 [Access: March 30, 2021]. doi:10.31838/srp.2020.9.76 AMA (American Medical Association) Style , Masrianih , Sofiana MSJ, Safitri I, Sapar A, Aritonang AB, Muttalib YS, Fadly D. Protein Isolation from Sponge Niphates sp. as an Antibacterial and Antioxidant. SRP. 2020; 11(9): 518-521. doi:10.31838/srp.2020.9.76 Vancouver/ICMJE Style , Masrianih , Sofiana MSJ, Safitri I, Sapar A, Aritonang AB, Muttalib YS, Fadly D. Protein Isolation from Sponge Niphates sp. as an Antibacterial and Antioxidant. SRP. (2020), [cited March 30, 2021]; 11(9): 518-521. doi:10.31838/srp.2020.9.76 Harvard Style , Masrianih, ., Sofiana, . M. S. J., Safitri, . I., Sapar, . A., Aritonang, . A. B., Muttalib, . Y. S. & Fadly, . D. (2020) Protein Isolation from Sponge Niphates sp. as an Antibacterial and Antioxidant. SRP, 11 (9), 518-521. doi:10.31838/srp.2020.9.76 Turabian Style , Masrianih, Mega Sari Juane Sofiana, Ikha Safitri, Ajuk Sapar, Anthoni B. Aritonang, Yuges Saputri Muttalib, and Dzul Fadly. 2020. Protein Isolation from Sponge Niphates sp. as an Antibacterial and Antioxidant. Systematic Reviews in Pharmacy, 11 (9), 518-521. doi:10.31838/srp.2020.9.76 Chicago Style , Masrianih, Mega Sari Juane Sofiana, Ikha Safitri, Ajuk Sapar, Anthoni B. Aritonang, Yuges Saputri Muttalib, and Dzul Fadly. "Protein Isolation from Sponge Niphates sp. as an Antibacterial and Antioxidant." Systematic Reviews in Pharmacy 11 (2020), 518-521. doi:10.31838/srp.2020.9.76 MLA (The Modern Language Association) Style , Masrianih, Mega Sari Juane Sofiana, Ikha Safitri, Ajuk Sapar, Anthoni B. Aritonang, Yuges Saputri Muttalib, and Dzul Fadly. "Protein Isolation from Sponge Niphates sp. as an Antibacterial and Antioxidant." Systematic Reviews in Pharmacy 11.9 (2020), 518-521. Print. doi:10.31838/srp.2020.9.76 APA (American Psychological Association) Style , Masrianih, ., Sofiana, . M. S. J., Safitri, . I., Sapar, . A., Aritonang, . A. B., Muttalib, . Y. S. & Fadly, . D. (2020) Protein Isolation from Sponge Niphates sp. as an Antibacterial and Antioxidant. Systematic Reviews in Pharmacy, 11 (9), 518-521. doi:10.31838/srp.2020.9.76 |
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