Synthesis And Anticancer Activity Evaluation Of Novel Ligand 2- [2 - (5-Chloro Carboxy Phenyl) Azo] 1-Methyl Imidazole (1-Mecpai) With Some Metal Complexes
Makarim A. Mahdi, Layth S. Jasim and Moslem H. Mohamed.
This work included synthesis of novel ligand 2- [2 - (5-Chloro carboxy phenyl) azo] 1-methyl imidazole (1-MeCPAI) was prepared by reacting a diazonium salt solution of 2-amino-4-chloro benzoic acid with 1-methyl imidazole. Also, preparation of a new series of chelate complexes of Nickel (II), Copper (II) and Zinc (II). Compounds have been characterized by UV-visible, FT-IR, 1H-NMR, 13C-NMR, magnetic susceptibility, Mass spectra, FE-SEM and XRD diffraction studies. We then studied biological activity in two groups of bacteria for all prepared compounds; Klebsilla pneumonia, Staphylococcus and Alternaria alternata as antifungal. The ligand (1-MeCPAI) also performed anticancer activity, Nickel complex and Copper complex by cells cytotoxicity and screen for in vitro antitumor activity against cancer of prostate line PC3. were evaluated using a colorimetric (MTT) test to determine their cellular viability. Copper complexes have especially shown selective cytotoxicity, destroying the cancer cell lines while not affecting the normal cells that are considered the essential for cytotoxic therapy. Tests have therefore carried out, which are designed to decide if this compound can be used to generate anti-cancer medicines in the future.