Synthesis, Characterization and Biological Activity of Zinc Oxide Nanoparticles (ZnO NPs)

Abstract

Asmaa H. Hammadi, S.Abdulmunem Habeeb, Lena Fadhil Al-Jibouri, Falah H. Hussien.

The main target of this paper, synthesis ZnO Nanosize by rapid easy and Successful method as Nano technique, study the structural ,optical and antibacterial activity of ZnO NPs catalyst .The ZnO NPs had been synthesized by thermal-precipitation method. This method achieved in KOH (pH=8) as alkaline media was important condition in precipitation method, in 60-70oC to provide thermal factor. Then the precipitate filtrated, washed and dried in air room temperature for 48h.later the catalyst calcined in 300 and 500oC in electric furnace for 120 min. Part of precipitate don’t treatment in furnace. The production was white nano powder characterized by SEM, EDX, and XRD.
The key aim of the current study is the investigation of antibacterial activity of ZnO NPs towards bacterial strains such as Gram-positive Staphylococcus aureus, Gram-negative Escherichia coli (E.coli) and Pseudomonas aeruginosa. This antibacterial activity was performed through the agar well diffusion method using different preparation temperatures of ZnO NPs, both with and without antibiotic (Oxytetracylin OTC). The resulting data showed that a relatively higher inhibiting zone was found in Gram-positive Staphylococcus aureus than for Escherichia coli and Pseudomonas aeruginosa, indicating that ZnO NPs do have antibacterial activity and could thereby improve the exact type of activity in antibiotics. It is therefore found that this study could be of use in formulating nano-drug conjugates that function as an antimicrobial agent within different aspects of research in medicine and pharmaceutics. `