The Antioxidant Effect of Indonesian Propolis In Rats Induced by Anthrax Spores

Abstract

Dhani Redhono, Bambang Purwanto, Brian Wasita, Dono Indarto, Rahmat Setya Adji, Arie Kusumawardani, Risya Cilmiaty

Background: Anthrax is a zoonotic infection caused by the pathogenic Bacillus anthracis which causes multi-organ dysfunction and necrosis of the target organ. Anthrax infection causes oxidative stress which is characterized by the formation of ROS that continues to endothelial dysfunction and tissue necrosis. Current management of anthrax using antibiotic arises problems related to the side effects of these antibiotics.
Objective: This study aims to prove the effect of Indonesian propolis as an antioxidant in rats induced by anthrax spores.
Materials and methods: This is a true experimental post-test only control group study on male white rats Rattus norvegicus, which were induced with anthrax spore and given ethanolic extracts of propolis (EEP) from Mount Lawu, Indonesia. Antioxidant effect was examined using ELISA on serum SOD as internal antioxidant and ROS level was assessed by serum MDA levels.
Results and Discussion: This study used 40 male rats which were divided into 5 treatment groups. SOD levels decreased in the group without EEP, while the group given EEP before induction showed the highest levels of SOD. Serum MDA levels in the Bacillus anthracis spore-induced group showed the highest yield, while the group given EEP and/or without antibiotics showed lower levels. The results of the correlation test for these two variables showed p ≤0.001.
Conclusion: Indonesian propolis has been shown to reduce ROS and increase internal antioxidants in rats induced by B. anthracis spore and proven to reduce the level of inflammation in lung tissue.