The cytotoxicity effects of liable and stable enterotoxins produced by uropathogenic Escherichia coli

Abstract

Muthanna Hamid Hassan

Objective: The aim of this study, detection of the stable and labile enterotoxins isolated from uropathogenic Escherichia coli and carry out cytotoxicity on cancer cell lines .
Methods: through the period extending from February 2019 till June 2019, 50 specimens of urine were collected from patients with urinary tract infections from two hospitals in Anbar and were collected in sterilized containers. Identification of E. coli was performed according to Bergy’s Manual for Determinative Bacteriology, and API 20E system was used and confirmed by vitek2. Extraction of DNA was carried out by using Plasmid DNA kit (Gene aid, Taiwan) and PCR reaction was used. Two primers were utilized to detect the lt and st encoding genes. cytotoxicity Performed by MTT.
Results: Out of 50 samples, 22 were not E. coli (44%), while 28 were detected as E. coli (56%).Infection with the tested bacteria appeared in 6 patients out of 28 and they were males (21.43%), whereas, females were 22 (78.57%). The st gene was found in two isolates (7.14%), while no isolates were carrying lt gene. The supernatant of bacteria contained biological activity such as anticancer effects against human cancer cell lines such as HeLa, RD and normal cell line (REF).
Conclusion, it was clearly demonstrated that stable toxins had high inhibition ability against cancer cell lines .