The Effect of Follicular Fluid Supplementation on Embryo Development in Iraqi Buffalo

Abstract

Husamaldeen A. Alsalim, Ihsan A. Habeeb, Ahzan K. Abdulameer

The study was carried out to evaluate the effect of supplementation of the buffalo follicular fluid (buFF) in culture medium (Ham’s F-10) on the development of oocytes in vitro maturation (IVM) rate, oocytes in vitro fertilization (IVF) rates, and embryonic developmental rate. The experimental study extended from November 2019 to March 2020. Samples included (80) female ovary and (20) bull testes was a collection from Basrah center slaughterhouse after 20 minutes from slaughter. Harvested samples were transported by disinfected clean box at (37 ºC) in less than 1 hrs to the lab. Only A and B grads quality oocytes were selected and incubated in a prepared maturation medium. Spermatozoa were obtained from slicing caudal of epididymal. Matured oocytes and capacitated sperms were incubated fertilization medium. Fertilize oocytes were re-incubated in fertilization medium with changing 50% of the medium every day followed by an embryonic developmental assessment for 4 days. Results illustrated a significant increase in oocytes maturation rate in Ham F-10 media with a 20% buFF group compared control group. On the other hand, this study showed a significant increase in fertilization rate in Ham’s F-10 medium with a 20% buFF group compared with the control group. Moreover, a high significant difference in embryonic development (2, 4,8,16 cells) in Ham’s F-10 media with 20% buffalo follicular fluid group compared with the control group. Thus, It has been concluded that supplementation of 20% buFF to media could be enhanced in vitro oocyte maturation, fertilization, and in vitro embryonic development in buffalo.