The Effect of Human Cytomegalovirus on IL-18 level in Iraqi Kidney Failure Patients

Abstract

Dheyaa Mohammad Ahmed Karalos and Raghad H. Al-azzawi

Kidney failure disease (CKD) represents an international major public anxiety, and its prevalence continues to rise. In order to study the effect of human cytomegalovirus (HCMV) on IL-18 levels in KF patients and compared with healthy control, the current study was designed. It was conducted from first December 2019 to the end of September 2020. Detection of HCMV (IgG/IgM) and IL-18 levels were assayed (by ELISA technique) in the sera of 70 chronic kidney failure patients and 70 healthy control. The patients' samples 44(62.86%0) males and 26(37.14%) females were collected from (dialysis units) of Baghdad Teaching Hospital, AL-Kindy Teaching Hospital, House nurses Hospital, AL-Emam Ali Hospital, and AL-Kadhimiya Teaching Hospitals. Seventy (70) healthy individuals were selected randomly (without kidney disease and HCMV infection), that include 53(75.71%) males and 17(24.29%) females. Age ranges were between (30-75 years) to each group (patient and control). All patients' samples were showed seropositive of HCMV-IgG and only 7 patients with anti HCMV-IgM positive. Serum level of IL-18 was increased in CK patients (0.109 ±0.009 pg/ml) as compared with control (0.071 ±0.003 pg/ml). The present result revealed that IL-18 was upregulated in kidney failure infected with Anti-HCMV patients, an observation that suggests their involvement in the pathogenesis of it.