The Effect of Stromal Vascular Fraction on Fracture Healing with Bone Defect: Experimental Study on Rattus Novergicus

Abstract

Panji Sananta, Respati Suryanto Dradjat, Istan Irmansyah Irsan, Domy Pradana Putra, Rizqi Daniar Rosandi, Adam Irsyaddyra, Lasa Dhakka Siahaan

Introduction: Bone defect is a condition whereby the bone tissue cannot undergo a natural healing process caused by severe trauma with significant tissue loss, tumor, or irradiation. Bone defect is a challenge even for experienced Orthopedic surgeons. Bone tissue engineering (BTE) is one of the methods pursued to manage this problem. One of the examples of BTE is the application of stromal vascular fraction (SVF). This study aims to evaluate the effect of SVF application on bone defect healing evaluated by radiographic study. Materials and Methods: This is an animal study involving 12 Wistar strain Rattus Norvegicus. They were divided into three groups: Negative control group (normal rats), Positive control group C2 (rats with bone defect without SVF and scaffold application), and Treatment group (rats with bone defect with SVF application). After 30 days, the rats were sacrificed, and the bone was evaluated radiographically. Results: Callus formation is best observed radiographically in the treatment group. All comparisons of SVF and positive control group showed a significant difference (p<0.05). Conclusion: Administration of SVF could aid bone defect healing through improving callus formation, marked by increased radiographic callus formation.