The Effect of Temperature and Storage Time on the Concentration of Vitamin D in Human Serum

Abstract

Asmaa Y. Al-Baitai, Saifaldeen M. Abdalhadi, Suhad A. Ibrahim, Ali A. Ali, Ghassan H. Alzamily

Vitamin D is one of the most important types of vitamins that are soluble in fats. The concentration of this vitamin must be maintained in order to work for the immune system, metabolism and skeletal health. The increase or decrease in the concentration of this vitamin in the blood directly affects the health system of men and women at all ages. In this research, 22 blood samples were examined at different times and temperatures in four cases. The first two cases, vitamin D was stored at 40 ?C and 25 ?C and tested every day within one week. The second two cases, when vitamin D was stored at 0 ?C and -8 ?C for 12 weeks and tested every two weeks and it was noted that the average vitamin D concentration at 40 ?C and 25 ?C for 7 days decreased to ~ 14% and ~ 2 % Respectively, while the mean concentration at 0 ?C and -8 ?C for 12 weeks decreased to ~ 22% and ~ 28%, respectively. These results revealed that 25 ?C is the best temperature for storing vitamin D in liquid form for one week, and that the concentration of vitamin D is directly affected when it is stored in high temperatures (40 ?C) or when it is frozen for long periods