The Effect of the Combination of Demineralization Freeze Dried Dentin Matrix (DFDDM) and Moringa oleifera Lam to increase Fibroblas and Osteoblas cell in Alveolar Bone after Caviacobaya?s Tooth Extraction

Abstract

Arni Irawaty Djais, Sri Oktawati, Hasanuddin Thahir, Muchammad Hatta, Bayu Indra Sukmana, Nurdiana Dewi, Huldani, Yayah Inayah, Widodo, Irene Edith Riewpassa, Harun Achmad, Dewiayu Dewang

Background: The socket preservation has important role in alveolar bone resorption after tooth extraction. Various graft materials can be used in socket preservation must have osteoconductive, osteoinductive and osteoproliferation properties. Autogenous bone graft from dentin can be used because it has good osteoinductive and osteoconductive properties. One of the active substances of MoringaoleiferaLam is flavonoidsthat have several beneficial characteristics as an anti?inflammatory. The combination of antiinflammatory with MO extract and Demineralization Freeze Dried Dentin Matrix (DFDDM) is expected to provide a good response to bone formation. Aim: To determine the potentialof combination MO and DFDDM in formation of Fibroblast and Osteoblast’s cells in post extraction caviacobaya’s tooth. Method: The mandibular incisors of 45 Caviacobaya were extracted and divided into five groups subjected to different socket preservation treatments sequentially with MO, DFDDM, combination of MO and DFDDM, Gamacha® and polyethylene glycol (PEG). The caviacobaya were examined on days 7, 14 and 21 after which the specimens were sacrificed and examined using an immunohistochemical technique. The resulting data were then analyzed using one?way ANOVA and Tukey’s honestly significant difference tests. Result: The One Way Anova test results showed a significant difference in FIbroblast and osteoblast between the groups (p<0.05) on day 7, 14 and 21 observation. The highest mean amount of OPG and lowest mean amount of RANKL were found in the third group. Conclusion: Combination of MO and DFDDM can effectively generate Fibroblast and osteoblast expressions during the preservation of tooth extraction sockets.