The Immunomodulatory Activity In-vitro of NDV ZG1999HDS in Comparison to NDV La Sota
The immunotherapy’s, as a modern therapeutic approach, get attention because of their potential to treat a large number of different medical disorders. Immunomodulation effects of low titres (10 ha/ml) of NDV (Newcastle Disease Virus) ZG1999HDS or La Sota were tested on TLT (Human macrophage cell line) bound to PBMC (Peripheral Blood Mononuclear Cells). During the immunomodulation, the amount of NO, H2O2, lysozyme and induced antibacterial activity against gram-positive bacteria (Staphylococcus aureus, Methicillin-Resistant Staphylococcus aureus (MRSA), Streptococcus pyogenes, Streptococcus agalactiae and Streptococcus mutants) and against gram-negative bacteria (Escherichia coli, Pseudomonas aeruginosa, Proteus mirabilis and Acinetobacter baumanii) were analysed. In addition, the secretion of cytokines Interleukin 1 alpha (IL-1 α), IL-2, IL-4, Granulocyte Macrophage Colony-Stimulating Factor (GM-CSF), Tumor necrosis factor (TNF-α), Interferons (IFN-α) and IFN-γ was evaluated. First, the TLT cells are activated through the NDV ZG1999HDS or La Sota binding, followed by the NO “burst” and H2O2 and lysozyme level increase. Then, after the binding to the TLT cells and interaction with the PBMCs, the decrease of GM-CSF and an increase of TNF-α and IFN-γ were found. Simultaneously, the decrease of pro-inflammatory cytokine IFN-α, and the differential increase of IL-1 α, IL-2 and IL-4 were observed. During the induction of the antibacterial response, against gram-positive bacteria (Staphylococcus aureus, MRSA, Streptococcus pyogenes, Streptococcus agalactiae and Streptococcus mutants) the effect was one third higher with NDV ZG1999HDS compared to La Sota. Antibacterial response against gram-negative bacteria (Escherichia coli, Pseudomonas aeruginosa, Proteus mirabilis and Acinetobacter baumanii) was not so clear. In general, NDV ZG1999HDS or La Sota activated TLT cells, further bound to PBMC; the ZG1999HDS is stronger immunomodulator than La Sota.