The Oxidative Stress Induced by the Vapours of Electronic- Hookah on Mice Liver Tissues

Abstract

Rasha Shaker Nima, Dhifaf Zeki Aziz.

Background: In the past few years, the increased use of Electronic- hookah is popular among youth in Iraq, raises the need for assessment of these products on liver tissue oxidative stress. The current study aims to examine the oxidative stress induced by the vapours of Electronic- hookah on mice liver tissues.
Material and Methods: Twenty mice were randomly separated into two groups. The first experimental group included albino mice were subjected to vapours of Electronic- hookah (1 hour per day for one month), and the other as a control group. At the end of the experiment, several organ weights were measured, and liver tissues were collected for all animals. Liver tissue contents of malondialdehyde, total protein carbonyl, nitric oxide were estimated.
Results: The experimental data showed that Electronic- hookah (1 hour per day for one-month) had significant differences between the two groups on the relative weight of the liver and relative weight of spleen. All of the estimated oxidative damage products (malondialdehyde, total protein carbonyl, nitric oxide) were significantly higher (P<0.0001) in the E- hookah group when compared to the animals in control group.
Conclusion: Vapours of Electronic- hookah have a harmful toxic effect that can promote worse oxidative stress, especially on liver tissue, which could elevate the risk for the development of liver diseases in a long time.