paper.io

agar io

wowcappadocia.com cappadocia tours
cappadocia-hotels.com cappadocia hotels
caruscappadocia.com cappadocia hotel
brothersballoon.com cappadocia balloon
balloon-rides.net cappadocia balloon flights

wormax io

rolex replica

Characterization of Viral Gene Proteins Panleukopenia Local Isolate as a Vaccine Candidate

Abstract

Muhammad Munawaroh, Mohammad Sukmanadi, Aulanni’am, Fedik Abdul Rantam

The purpose of this study was to determine the genetic characteristics of local FPV isolates in Indonesia as a basis for determining the type of FPV to be used as a panleukopenia vaccine material in Indonesia. This research was conducted in March - September 2019. Rectal swab or stool samples were taken, performed PCR and Sequencing, data were analyzed using the BioEdit program, homology analysis and phylogenetic trees using MEGA software. The results of this study found FPV isolates using PCR. In addition, based on the alignment results, the matching results of the sample sequences V1 and V2, respectively, are the Felocell® 3 vaccine and Purevax RCP. Whereas FPV virus field isolates namely V3 - V8 samples showed some differences in sequences namely in sequences 5, 95, 150, 173, and 425. Differences in nucleotide bases in several strains, vaccines and field isolates also showed a clear genetic difference in phylogenetic trees. Alignment of DNA sequences obtained homogeneous in several strains of FPV according to the NCBI database, which is precisely in the VP1 protein, VP2 FPV. The results of sequencing from eight samples showed a match on eight strains of FPV virus in a database with identities ranging from 99.2% - 99.8%. The conclusion from the results of this study is that FPV isolates were obtained and could be used as vaccine candidates for FPV in Indonesia. In addition, further studies on experimental animals are needed.

Most Viewed Articles
  • Dental Development between Assisted Reproductive Therapy (Art) and Natural Conceived Children: A Comparative Pilot Study Norzaiti Mohd Kenali, Naimah Hasanah Mohd Fathil, Norbasyirah Bohari, Ahmad Faisal Ismail, Roszaman Ramli SRP. 2020; 11(1): 01-06 » doi: 10.5530/srp.2020.1.01
  • Psychometric properties of the World Health Organization Quality of life instrument, short form: Validity in the Vietnamese healthcare context Trung Quang Vo*, Bao Tran Thuy Tran, Ngan Thuy Nguyen, Tram ThiHuyen Nguyen, Thuy Phan Chung Tran SRP. 2020; 11(1): 14-22 » doi: 10.5530/srp.2019.1.3
  • A Review of Pharmacoeconomics: the key to “Healthcare for All” Hasamnis AA, Patil SS, Shaik Imam, Narendiran K SRP. 2019; 10(1): s40-s42 » doi: 10.5530/srp.2019.1s.21
  • Deuterium Depleted Water as an Adjuvant in Treatment of Cancer Anton Syroeshkin, Olga Levitskaya, Elena Uspenskaya, Tatiana Pleteneva, Daria Romaykina, Daria Ermakova SRP. 2019; 10(1): 112-117 » doi: 10.5530/srp.2019.1.19
Most Downloaded
  • Dental Development between Assisted Reproductive Therapy (Art) and Natural Conceived Children: A Comparative Pilot Study Norzaiti Mohd Kenali, Naimah Hasanah Mohd Fathil, Norbasyirah Bohari, Ahmad Faisal Ismail, Roszaman Ramli SRP. 2020; 11(1): 01-06 » doi: 10.5530/srp.2020.1.01
  • Manilkara zapota (L.) Royen Fruit Peel: A Phytochemical and Pharmacological Review Karle Pravin P, Dhawale Shashikant C SRP. 2019; 10(1): 11-14 » doi: 0.5530/srp.2019.1.2
  • Pharmacognostic and Phytopharmacological Overview on Bombax ceiba Pankaj Haribhau Chaudhary, Mukund Ganeshrao Tawar SRP. 2019; 10(1): 20-25 » doi: 10.5530/srp.2019.1.4
  • A Review of Pharmacoeconomics: the key to “Healthcare for All” Hasamnis AA, Patil SS, Shaik Imam, Narendiran K SRP. 2019; 10(1): s40-s42 » doi: 10.5530/srp.2019.1s.21
  • A Prospective Review on Phyto-Pharmacological Aspects of Andrographis paniculata Govindraj Akilandeswari, Arumugam Vijaya Anand, Palanisamy Sampathkumar, Puthamohan Vinayaga Moorthi, Basavaraju Preethi SRP. 2019; 10(1): 15-19 » doi: 10.5530/srp.2019.1.3