Characterization of Viral Gene Proteins Panleukopenia Local Isolate as a Vaccine Candidate

Abstract

Muhammad Munawaroh, Mohammad Sukmanadi, Aulanni’am, Fedik Abdul Rantam

The purpose of this study was to determine the genetic characteristics of local FPV isolates in Indonesia as a basis for determining the type of FPV to be used as a panleukopenia vaccine material in Indonesia. This research was conducted in March - September 2019. Rectal swab or stool samples were taken, performed PCR and Sequencing, data were analyzed using the BioEdit program, homology analysis and phylogenetic trees using MEGA software. The results of this study found FPV isolates using PCR. In addition, based on the alignment results, the matching results of the sample sequences V1 and V2, respectively, are the Felocell® 3 vaccine and Purevax RCP. Whereas FPV virus field isolates namely V3 - V8 samples showed some differences in sequences namely in sequences 5, 95, 150, 173, and 425. Differences in nucleotide bases in several strains, vaccines and field isolates also showed a clear genetic difference in phylogenetic trees. Alignment of DNA sequences obtained homogeneous in several strains of FPV according to the NCBI database, which is precisely in the VP1 protein, VP2 FPV. The results of sequencing from eight samples showed a match on eight strains of FPV virus in a database with identities ranging from 99.2% - 99.8%. The conclusion from the results of this study is that FPV isolates were obtained and could be used as vaccine candidates for FPV in Indonesia. In addition, further studies on experimental animals are needed.