Detection of Metallo β-lactamase in Klebseilla pneumoniae Isolated From Upper Respiratory Tract of Carrier individuals In Iraq
Nadia Abdulrazzaq Jamal, Mohammed Nuh AL-Khafaf and Saladdin M. Abdulazeez
Klebsiella pneumoniae is an a health care challenge over all the world, due to its resistance to many effective antibacterial, particularly those depend as a standard therapeutic options, Klebsiella carbapenemase producing have been emerged in Indian provinces, this phenomenon then have been disseminated in many countries around the world. The most successful Î²-lactam hydrolyzing enzymes are belonging to the above antibiotic category is New Delhi metallo Î²-lactamase, presence of NDM is an indication of multi-drug resistance. K.pneumoniae was isolated from Indian employees in Erbil city and from Iraqi people living in Mosul city, who had previously conducted an interventional surgery at hospitals in India.Of a total of 35 K.pneumoniae isolates, 6 KCP strains were discovered by phenotypic analysis using the modified Hudge test. While molecular screening showed 3 KCP as a true positive result. High molecular sensitivity and specificity, based on Gradiant Multiplex PCR, were found in the comparison with classical diagnosis method.On the other hand, according to the Random Amplification Polymorphic DNA, genetic variation has categorized the six KCP isolates into three clusters due to the existence and absence of random amplicons.