Granulicatella Adiacens Bacteria Isolation from Perodontitical Patients with Polymerase Chain Reaction Techniques

Abstract

Harun Achmad, Sri Oktawati, Andi Mardiana Adam, Burhanuddin Pasiga, Rizalinda Sjahril, Aulia Azizah, Bayu Indra Sukmana, Huldani, Heri Siswanto, Ingrid Neormansyah

Background: Periodontitis is an inflammatory disease of dental support tissue caused by certain groups of microorganisms, resulting in progressive destruction of the periodontal ligament and alveolar bone, with pocket formation, recession, or both1 . Destruction of periodontal caused by the host defence. Objective: Granulicatella adiacens is a small Gram-positive, non-motile, non-spore-forming, facultatively anaerobic coccus. As a member of Nutritionally Variant Streptococci, genus Granulicatella, it is a part of the normal microbiota in the oral cavity, urogenital, and intestinal tract. It may also participate in periodontitis, caries, and endodontic infections. Our aim in this study was to investigate the presence of Granulicatella adiacens in periodontal disease Methods: A total 18 samples from patients with chronic periodontitis was collected, by using the conventional PCR technique for checking the presence of bacterial bands, followed by using DNA sequence methods, to complete the examination of bacteria with Conventional PCR which results are used as a basis for nucleotide examination on the NCBI Gene Bank using BLAST analysis. Result: In this study, Granulicatella adiancense was found in 13 samples from 18 solated samples from patients with chronic periodontitis and in periodontal pocket depth more than 7 mm. Conclusion: Granulicatella adiancense is a normal flora bacteria in the oral cavity and chronic periodontitis conditions, the presence of this bacteria is detected in the deeper periodontal pocket depth.