Polymerase Chain Reaction (PCR) as a Rapid Examination Accompanied with Bacterial Culture to Recognition of Taylorella equigenitalis in Infertility Mares of the Northwest of Iran

Abstract

Aref Aalinasab57742, Esmail Ayen57743*, Katayun Nofouzi57744 and Ali Soleimanzadeh Azad57745

Taylorella equigenitalis is the causative agent of Contagious Equine Metritis (CEM) and is a severe epidemic that causes interim infertility in the horse nurture industry. Nowadays, the Polymerase Chain Reaction (PCR) technique is applied to detect these bacteria as one of the most sensitive and the fastest methods. In the current study, T. equigenitalis was followed by sampling from northwest of Iran in summer 2021 in clitoris sinusoidal-fossa and urinary tract in 210 mares and 30 stallions. Mares’ age range was 3 to 20 years old and stallions’ age range was 4 to 10 years old. Two swabs one for culture, and the other was used for PCR in equine clitoris sinusoidal and mares’ urinary tract sinus. They were transferred to the veterinary microbiology laboratory of the University of Tabriz. In received samples, investigation with bacterial culture method in chocolate agar medium and one-week presence in CO2, the examination was not successful; but we observed three positive samples from 240 cases (3 mares) with the genotypic method after DNA extraction and PCR in received samples for studied horses of Ardabil province and one sample (mare) was positive in West Azerbaijan. East Azerbaijan didn’t have a positive sample among 80 cases. This study shows that the rapid method for recognition of T. equigenitalis by using PCR is based on bacterial genome 16S rDNA part and it is a special technique with high-level features against culturing cases.