Assessment of Pain-Ameliorative Potential of Ethanolic Extract of Calotropis procera and its Active Components characterization by HPTLC and GC-MS Analysis
Introduction: Calotropis procera is a shrub had been used traditionally for the treatment of many diseases including analgesic, anti-inflammatory, hepatic and other diseases. The flower of the plant has not been investigated for its analgesic activity. The present study aims High Pressure Thin Layer Chromatography (HPTLC) and Gas Chromatography-Mass Spectrometry (GC-MS) analysis of Ethanolic Extract of Calotropis procera to evaluate the analgesic effects of Calotropis procera as well as assessment of analgesic activity in animal models.
Methods: The ethanolic extracts were prepared using a rotary evaporator and the maceration procedure. The phytocompounds characterization was done by GC-MS and HPTLC. The hot plate method, acetic acid- induced writhing test, and tail-flick test were used to determine the analgesic activity of various doses (100, 200, 300 mg/kg body weight) of the Ethanolic Extract of Calotropis procera flower (EECP).
Conclusion: The extract has shown showed significant analgesic activity in the all experiments at a dose of 300 mg/kg. We found that even at low doses, analgesic effectiveness could be achieved via central and peripheral mediated action. The ethanol extract’s HPTLC and GC-MS analysis revealed number of compounds with potent analgesic and antioxidant activity.